OneTaqQuick-Load 2X Master Mix with Standard Buffer from New England Biolabs

Product Specs
ItemOneTaqQuick-Load 2X Master Mix with Standard Buffer
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0486S
Concentration2 X
Quantity100 reactions
ApplicationsDNA Analysis
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeMaster Mix
Temperature-20 C

Specifications/Features

Information:
Source: An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent_R DNA Polymerase gene from Pyrococcus species GB-D.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Nonmonotone invasion landscape by noise-aware control of metastasis activator levelsNature Chemical BiologyJuly 1, 2023Yiming Wan, Joseph Cohen, Mariola Szenk, Kevin S. Farquhar, Damiano Coraci, Rafał Krzysztoń, Joshua Azukas, Nicholas Van Nest, Alex Smashnov, Yi-Jye Chern, et al.with OneTaq DNA Polymerase (OneTaq Quick-Load 2× Master Mix with Standard Buffer, New England Biolabs, M0486S) in 25-μl reactions. Primer sequences for constructs in Extended Data Fig. 1a,f are listed in Supplementary

(2) Ectopic Rod Photoreceptor Development in Mice with Genetic Deficiency of WNT2BCellsMarch 28, 2023Blomfield AK, Maurya M, Bora K, Pavlovich MC, Yemanyi F, Huang S, Fu Z, O'Connell AE, Chen J.PCR was performed using OneTaq Quick-Load 2X master mix (New England BioLabs, Ipswich, MA, USA, Cat#: M0486S) and followed protocols described previously [18,19,24]. For cre genotyping, PCR was performed with

(3) Bacterial genome engineering using CRISPR RNA-guided transposasesbioRxivMarch 25, 2023Diego R. Gelsinger, Phuc Leo H. Vo, Sanne E. Klompe, Carlotta Ronda, Harris Wang, Samuel H. Sternbergpurchased separately: NEB cat. no. B9027S)(Optional) OneTaq Quick-Load 2X Master Mix (NEB cat. No. M0486S/L), for genotyping onlyOligonucleotide primers for PCR, from IDT or preferred vendorSsoAdvanced Universal

(4) Alternative splicing modulation by G-quadruplexesNature CommunicationsDecember 1, 2022Ilias Georgakopoulos-Soares, Guillermo E. Parada, Hei Yuen Wong, Ragini Medhi, Giulia Furlan, Roberto Munita, Eric A. Miska, Chun Kit Kwok, Martin HembergGCTCACTCTCTTCCGCATC and PI12_R: CTTGGCGTTCGGAGGATG using One TaqR DNA polymerase (New England BioLabs #M0486S) and run on a thermocycler with the following conditions: (1) Initial denaturation 2’ at 94 °C; (2)

(5) Antidiabetic activity of avocado seeds (Persea americana Mill.) in diabetic rats via activation of PI3K/AKT signaling pathwayScientific ReportsDecember 1, 2022Oluwafemi Adeleke Ojo et al.primer (100 pmol) 12.5 µl Ready Mix Taq PCR master mix (One Taq Quick-Load 2x, master mix, NEB, Cat: M0486S) and 8.5 µl nuclease-free water. Early denaturation at 95 °C for 5 min was followed by 20 cycles of

(6) Annona muricata L. peel extract inhibits carbohydrate metabolizing enzymes and reduces pancreatic Î²-cells, inflammation, and apoptosis via upregulation of PI3K/AKT genesPLOS ONEOctober 27, 2022Oluwafemi Adeleke Ojo, Susan Grant, Jennifer Chidubem Amanze, Abosede Itunuoluwa Oni, Adebola Busola Ojo, Tobiloba Christiana Elebiyo, Tajudeen Olabisi Obafemi, Damilare Iyinkristi Ayokunle, Akingbolabo Daniel Ogunlakinenhancement was achieved. 12.5 μl Ready Mix Taq PCR master mix (One Taq Quick-Load 2x, master mix, NEB, Cat: M0486S) and 8.5 μl nuclease-free water. An initial denaturation at 95°C for 5 minutes was followed by 20 cycles

(7) RNA editing underlies genetic risk of common inflammatory diseasesNatureAugust 18, 2022Qin Li, Michael J. Gloudemans, Jonathan M. Geisinger, Boming Fan, François Aguet, Tao Sun, Gokul Ramaswami, Yang I. Li, Jin-Biao Ma, Jonathan K. Pritchard, et al.diluted cDNA, 1 μl was then amplified via PCR using OneTaq Quick-Load 2X Master Mix with Standard Buffer (M0486S, NEB) and primers designed to amplify from mClover3 through the CTSA 3′-UTR or from mRuby3 through the

(8) Protocols for marker-free gene knock-out and knock-down in Kluyveromyces marxianus using CRISPR/Cas9FEMS Yeast ResearchJanuary 29, 2022Arun S Rajkumar, John P MorrisseyFormedium). Molecular biology enzymes and reagents OneTaq® Quick-Load® 2X Master Mix with Standard Buffer (M0486S/L, New England Biolabs; Ipswitch, U.S.A.).Depending on availability and costs, DreamTaq Green PCR Master

(9) mRNA Vaccine Protects against Zika VirusVaccines (Basel)December 10, 2021Lex G. Medina-Magües, Janina Gergen, Edith Jasny, Benjamin Petsch, Jaime Lopera-Madrid, Emily S. Medina-Magües, Cristhian Salas-Quinchucua, Jorge E. Osorioand the primer ZIKV 1162c using OneTaq® Quick-Load® 2× Master Mix with Standard Buffer (NEB, Cat. No. M0486S). RNA was synthesized using MEGAscript T7 (Invitrogen, Cat. No. AM1334), TURBO DNAse-treated, and purified

(10) Synthetic Food dyes cause testicular damage via up-regulation of pro-inflammatory cytokines and down-regulation of FSH-R and TESK-1 gene expressionJBRA Assisted ReproductionJuly 21, 2021Iheanyichukwu Wopara, Emmanuel U. Modo, Samuel Kelechi Mobisson, Olusegun G. Olusegun, Elizabeth B. Umoren, Orji Blessing Orji, Philippe Mounmbegna Mounmbegna, Stephanie Okoye Stephanieprimer (100pmol) 12.5µl Ready Mix Taq PCR master mix (One Taq Quick-load 2x, master mix, NEB, Cat: M0486S) and 8.5µl nuclease-free water. Initial denaturation at 95˚C for 5 minutes was followed by 20 cycles

(11) Genetic variation in the bovine myogenic determination factor 1 (g.782G>A polymorphism) and its influence on carcass traits in Turkish Grey Steppe cattleTURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCESJune 29, 2021Sena ARDIÇLI, Özden ÇOBANOĞLUtemplate, 12.50 μL PCR master mix (OneTaq Quick-Load 2x MM, New England BioLabs Inc., Ipswich, MA, USA,Cat# M0486S), 1 μL of forward and reverse primers (0.5 μM), and 8 μL of distilled deionized water (ddH2O). The primers

(12) gbpA and chiA genes are not uniformly distributed amongst diverse Vibrio choleraeMicrobial GenomicsJune 8, 2021Thea G. Fennell, Grace A. Blackwell, Nicholas R. Thomson, Matthew J. Dormansuspension as a PCR template using primers oMJD204 and oMJD205 and OneTaq Quickload 2X Master Mix (NEB; #M0486S), according to the manufacturer’s instructions (annealing temperature 45 °C, extension time 3 min).

(13) Studies on Pancreatic Gene Expression in Diabetic Rats Treated with Moringa oleifera LeafJournal of Pharmaceutical Research InternationalMay 4, 2021Arvin Nwakulite et al.primer (100 pmol) 12.5 µl Ready Mix Taq PCR master mix (One Taq Quick-Load 2x, master mix, NEB, Cat: M0486S) and 8.5 µl nuclease-free water. Initial 81 Nwakulite et al.; JPRI, 33(28A): 78-86, 2021; Article

(14) Production of TRPM4 knockout cell line using rat cardiomyocyte H9c2MethodsXJanuary 1, 2021Wang C, Maeda M, Chen J, Wang M, Naruse K, Takahashi K.total) was prepared as follows: 25 µl OneTaq Quick-Load 2X Master Mix with Standard Buffer (Cat no: M0486S, New England Biolabs, Tokyo, Japan), 0.5 µl forward primer (20 µM), 0.5 µl reverse primer (20 µM), ~900

(15) Sub-acute toxicity study of methanol extract of Tetrorchidium didymostemon leaves using biochemical analyses and gene expression in Wistar ratsHeliyonJune 27, 2020Osamudiamen Ebohon, Francis Irabor, Ehimwenma Sheena Omoregieprimer (100 pmol) 12.5 μl Ready Mix Taq PCR master mix (One Taq Quick-Load 2x, master mix, NEB, Cat: M0486S) and 8.5 μl nuclease-free water. Initial denaturation at 95 °C for 5 min was followed by 20 cycles of

(16) A primal incrimination of Cedecea davisae with post-prostatectomy urinary tract infection in NigeriaInternational Journal of Biological and Chemical SciencesAugust 17, 2018D. E. Agbonlahor et al.TEM, SHV and aac (3)-II Amplification was carried out using OneTaq one-step PCR mix (catalog No. NEB M0486S), supplied by New England Bio Labs Incorporation (Massachusetts), according to the manufacturer’s specification.

(17) A Laboratory Study on the Molecular Basis of Primary Congenital GlaucomaJournal of Clinical Research and OphthalmologyJune 18, 2018UA UbanidCTP, 400μM dTTP and 3mM MgCl2. The New England Biolabs (NEB) (OneTaq quick load, 2X MM w/std buffer M0486S) contains dNTPs, MgCl2, Homo buffer components and stabilizers. The NEB was used due MF DOB 7/11/08

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